Quantitation of inflammatory und proliferative genes as disease markers in laser-microdissected, formalin-fixed and paraffinized glomeruli from human renal biopsies

2002 ◽  
Vol 3 (3-4) ◽  
pp. 1-11
Author(s):  
Jochen Walter Ulrich Fries ◽  
Alexandra Pakula ◽  
Tanja Roth ◽  
Hans-Peter Dienes ◽  
Margarete Odenthal
Keyword(s):  
Disease Markers ◽  
Renal Biopsies ◽  
Formalin Fixed

Quantitation of inflammatory und proliferative genes as disease markers in laser-microdissected, formalin-fixed and paraffinized glomeruli from human renal biopsies

2002 ◽  
Vol 3 (34) ◽  
pp. 98-108 ◽  
Author(s):  
Jochen Walter Ulrich Fries ◽  
Alexandra Pakula ◽  
Tanja Roth ◽  
Hans-Peter Dienes ◽  
Margarete Odenthal
Keyword(s):  
Disease Markers ◽  
Renal Biopsies ◽  
Formalin Fixed

scholarly journals Formalin-fixed paraffin-embedded renal biopsy tissues: an underexploited biospecimen resource for gene expression profiling in IgA nephropathy

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Sharon Natasha Cox ◽  
Samantha Chiurlia ◽  
Chiara Divella ◽  
Michele Rossini ◽  
Grazia Serino ◽  
...  
Keyword(s):  
Gene Expression ◽  
Iga Nephropathy ◽  
Clinical Decision Making ◽  
Expression Profiles ◽  
Specific Gene ◽  
Renal Lesions ◽  
Formalin Fixed Paraffin ◽  
Renal Biopsies ◽  
Formalin Fixed Paraffin Embedded ◽  
Formalin Fixed

Abstract Primary IgA nephropathy (IgAN) diagnosis is based on IgA-dominant glomerular deposits and histological scoring is done on formalin-fixed paraffin embedded tissue (FFPE) sections using the Oxford classification. Our aim was to use this underexploited resource to extract RNA and identify genes that characterize active (endocapillary–extracapillary proliferations) and chronic (tubulo-interstitial) renal lesions in total renal cortex. RNA was extracted from archival FFPE renal biopsies of 52 IgAN patients, 22 non-IgAN and normal renal tissue of 7 kidney living donors (KLD) as controls. Genome-wide gene expression profiles were obtained and biomarker identification was carried out comparing gene expression signatures a subset of IgAN patients with active (N = 8), and chronic (N = 12) renal lesions versus non-IgAN and KLD. Bioinformatic analysis identified transcripts for active (DEFA4,TNFAIP6,FAR2) and chronic (LTB,CXCL6, ITGAX) renal lesions that were validated by RT-PCR and IHC. Finally, two of them (TNFAIP6 for active and CXCL6 for chronic) were confirmed in the urine of an independent cohort of IgAN patients compared with non-IgAN patients and controls. We have integrated transcriptomics with histomorphological scores, identified specific gene expression changes using the invaluable repository of archival renal biopsies and discovered two urinary biomarkers that may be used for specific clinical decision making.

scholarly journals Direct Immunofluorescence and Immunohistochemistry in Diagnostics of Glomerulonephritis

2008 ◽  
Vol 8 (1) ◽  
pp. 12-19
Author(s):  
Zinaida Karasalihović ◽  
Ermina Iljazović ◽  
Dušan Ferluga ◽  
Elmir Čičkušić ◽  
Jasminka Mustedanagić Mujanović ◽  
...  
Keyword(s):  
Single Step ◽  
Direct Immunofluorescence ◽  
Immunofluorescence Method ◽  
Complement Components ◽  
Renal Biopsies ◽  
Immunoglobulin Deposits ◽  
And Control ◽  
Formalin Fixed ◽  
Morphologic Changes

The needle biopsies from 60 transplanted and native kidneys have been processed and a prospective analysis of pattern, intensity and distribution of immunoglobulin deposits (IgA, IgG and IgM) and complement components (C3c and C1q) identified in these lesions has been carried out by immunohistochemistry with three step immunoperoxidase, in the period from 2000 to 2004. Those deposits were previously detected and analyzed by immunofluorescence. The samples consisted of 30 renal biopsies, previously diagnosed with glomerulonephritis and positive immunofluorescence and 30 renal biopsies without morphologic changes and deposits on immunofluorescence. 78,7% of the analyzed samples showed the identical results of the deposits of immunoglobulin and components of the complement with both, immunohistochemistry and immunofluorescence method. Sensitivity of the immunohistochemistry method with three step immunoperoxidase for all analyzed immunoglobulin and complement components is high (0,93), while specificity for the same method is 0,79. Standardized method of the three step immunoperoxidase on the paraffin embedded, formalin fixed needle renal biopsies could successfully replace the immunofluorescence method in diagnostic of GN, with the emphasis on a follow up and control of each single step in the procedure of the method.

scholarly journals Immunoperoxidase and immunofluorescence on formalin-fixed, paraffin-embedded tissue sections versus immunofluorescence on frozen sections in the assessment of renal biopsies

2020 ◽  
Vol 30 (1) ◽  
pp. 8
Author(s):  
NasarYousuf Alwahaibi ◽  
RajaaMohammed Alsidiri ◽  
ThuraiyaAmer Alsinawi ◽  
WafaHamed Almalki ◽  
ShadiaSaid Alsinawi ◽  
...  
Keyword(s):  
Frozen Sections ◽  
Tissue Sections ◽  
Formalin Fixed Paraffin ◽  
Renal Biopsies ◽  
Formalin Fixed Paraffin Embedded ◽  
Formalin Fixed

Ultrastructural Localization of Acid Mucosubstance in Skeletal Muscle

1967 ◽  
Vol 25 ◽  
pp. 52-53 ◽  
Author(s):  
William J. Dougherty ◽  
Samuel S. Spicer
Keyword(s):  
Striated Muscle ◽  
Divalent Cations ◽  
Ultrastructural Localization ◽  
Major Elements ◽  
Frozen Sections ◽  
Thorium Dioxide ◽  
Iron Solution ◽  
Coupling System ◽  
Psoas Muscles ◽  
Formalin Fixed

In recent years, considerable attention has focused on the morphological nature of the excitation-contraction coupling system of striated muscle. Since the study of Porter and Palade, it has become evident that the sarcoplastic reticulum (SR) and transverse tubules constitute the major elements of this system. The problem still exists, however, of determining the mechamisms by which the signal to interdigitate is presented to the thick and thin myofilaments. This problem appears to center on the movement of Ca++ions between myofilaments and SR. Recently, Philpott and Goldstein reported acid mucosubstance associated with the SR of fish branchial muscle using the colloidal thorium dioxide technique, and suggested that this material may serve to bind or release divalent cations such as Ca++. In the present study, Hale's iron solution adapted to electron microscopy was applied to formalin-fixed myofibrils isolated from glycerol-extracted rabbit psoas muscles and to frozen sections of formalin-fixed rat psoas muscles.

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